American Journal of Kidney Diseases
Volume 54, Issue 5 , Pages 871-880 , November 2009

Very Low-Molecular-Mass Fragments of Albumin in the Plasma of Patients With Focal Segmental Glomerulosclerosis

  • Joan Lopez Hellin, PhD

      Affiliations

    • Fisopatologia Renal, CIBBIM, Institut de Recerca, Hospital Vall d'Hebron, Barcelona, Spain
    • Corresponding Author InformationAddress correspondence to Joan Lopez Hellin, PhD, Fisiopatologia Renal-CIBBIM, Lab 117, Institut de Recerca, Hospital Vall d'Hebron, P. Vall d'Hebron, 119-129, 08038 Barcelona, Spain
  • ,
  • Joan J. Bech-Serra, PhD

      Affiliations

    • Proteomics Laboratory, Institut de Recerca, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Enrique Lara Moctezuma, MD

      Affiliations

    • Pediatric Nephrology Department, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Sara Chocron, MD

      Affiliations

    • Pediatric Nephrology Department, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Sheila Santin, BS

      Affiliations

    • Molecular Biology Laboratory, Nephrology Department, Fundació Puigvert, Barcelona, Spain
  • ,
  • Alvaro Madrid, MD

      Affiliations

    • Pediatric Nephrology Department, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Ramon Vilalta, MD

      Affiliations

    • Pediatric Nephrology Department, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Francesc Canals, PhD

      Affiliations

    • Proteomics Laboratory, Institut de Recerca, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Roser Torra, MD, PhD

      Affiliations

    • Molecular Biology Laboratory, Nephrology Department, Fundació Puigvert, Barcelona, Spain
  • ,
  • Anna Meseguer, PhD

      Affiliations

    • Fisopatologia Renal, CIBBIM, Institut de Recerca, Hospital Vall d'Hebron, Barcelona, Spain
  • ,
  • Jose L. Nieto, MD

      Affiliations

    • Pediatric Nephrology Department, Hospital Vall d'Hebron, Barcelona, Spain

Received 10 December 2008 ,Accepted 23 July 2009.

References 

  1. Deegens JK, Dijkman HB, Borm GF, et al. Podocyte foot process effacement as a diagnostic tool in focal segmental glomerulosclerosis. Kidney Int. 2008;74:1568–1576
  2. Eddy AA, Symons JM. Nephrotic syndrome in childhood. Lancet. 2003;362:629–639
  3. Cameron JS. Focal segmental glomerulosclerosis in adults. Nephrol Dial Transplant. 2003;18(suppl 6):vi45–vi51
  4. Kitiyakara C, Eggers P, Kopp JB. Twenty-one-year trend in ESRD due to focal segmental glomerulosclerosis in the United States. Am J Kidney Dis. 2004;44:815–825
  5. Niaudet P. Genetic forms of nephrotic syndrome. Pediatr Nephrol. 2004;19:1313–1318
  6. Sharma M, Sharma R, McCarthy ET, Savin VJ. The focal segmental glomerulosclerosis permeability factor: biochemical characteristics and biological effects. Exp Biol Med (Maywood). 2004;229:85–98
  7. Daskalakis N, Winn MP. Focal and segmental glomerulosclerosis. Cell Mol Life Sci. 2006;63:2506–2511
  8. Carraro M, Zennaro C, Candiano G, et al. Nephrotic urine prevents increased rat glomerular albumin permeability induced by serum from the same patient with idiopathic nephrotic syndrome. Nephrol Dial Transplant. 2003;18:689–693
  9. Briggs JD, Jones E. Recurrence of glomerulonephritis following renal transplantation (Scientific Advisory Board of the ERA-EDTA Registry. European Renal Association-European Dialysis and Transplant Association). Nephrol Dial Transplant. 1999;14:564–565
  10. Crosson JT. Focal segmental glomerulosclerosis and renal transplantation. Transplant Proc. 2007;39:737–743
  11. Caridi G, Perfumo F, Ghiggeri GM. NPHS2 (podocin) mutations in nephrotic syndrome (Clinical spectrum and fine mechanisms). Pediatr Res. 2005;57:54R–61R
  12. Carraro M, Caridi G, Bruschi M, et al. Serum glomerular permeability activity in patients with podocin mutations (NPHS2) and steroid-resistant nephrotic syndrome. J Am Soc Nephrol. 2002;13:1946–1952
  13. Sharma M, Sharma R, McCarthy ET, Savin VJ. “The FSGS factor”: enrichment and in vivo effect of activity from focal segmental glomerulosclerosis plasma. J Am Soc Nephrol. 1999;10:552–561
  14. Le Berre L, Godfrin Y, Lafond-Puyet L, et al. Effect of plasma fractions from patients with focal and segmental glomerulosclerosis on rat proteinuria. Kidney Int. 2000;58:2502–2511
  15. Lopez-Hellin J, Gonzalo R, Tejeda M, et al. Transcriptomic and proteomic analysis of liver and muscle alterations caused by surgical stress in rat. Clin Sci (Lond). 2005;108:167–168
  16. Schagger H. Tricine-SDS-PAGE. Nat Protoc. 2006;1:16–22
  17. Janech MG, Raymond JR, Arthur JM. Proteomics in renal research. Am J Physiol Renal Physiol. 2007;292:F501–F512
  18. Thongboonkerd V, Malasit P. Renal and urinary proteomics: current applications and challenges. Proteomics. 2005;5:1033–1042
  19. O'Riordan E, Gross SS, Goligorsky MS. Technology insight: renal proteomics—at the crossroads between promise and problems. Nat Clin Pract Nephrol. 2006;2:445–458
  20. Gygi SP, Corthals GL, Zhang Y, Rochon Y, Aebersold R. Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc Natl Acad Sci U S A. 2000;97:9390–9395
  21. Quero C, Colome N, Prieto MR, et al. Determination of protein markers in human serum: analysis of protein expression in toxic oil syndrome studies. Proteomics. 2004;4:303–315
  22. Osicka TM, Comper WD. Characterization of immunochemically nonreactive urinary albumin. Clin Chem. 2004;50:2286–2291
  23. Hilliard LM, Osicka TM, Clavant SP, Robinson PJ, Nikolic-Paterson DJ, Comper WD. Characterization of the urinary albumin degradation pathway in the isolated perfused rat kidney. J Lab Clin Med. 2006;147:36–44
  24. Clavant SP, Sastra SA, Osicka TM, Comper WD. The analysis and characterisation of immuno-unreactive urinary albumin in healthy volunteers. Clin Biochem. 2006;39:143–151
  25. Candiano CBM, Petretto A, Santucci L, et al. Proteins and protein fragments in nephrotic syndrome: clusters, specificity and mechanisms. Proteomics Clin Appl. 2008;2:956–963
  26. Candiano G, Musante L, Bruschi M, et al. Repetitive fragmentation products of albumin and alpha1-antitrypsin in glomerular diseases associated with nephrotic syndrome. J Am Soc Nephrol. 2006;17:3139–3148
  27. Musante L, Bruschi M, Candiano G, et al. Characterization of oxidation end product of plasma albumin ‘in vivo’. Biochem Biophys Res Commun. 2006;349:668–673
  28. Candiano G, Santucci L, Petretto A, et al. High-resolution 2-DE for resolving proteins, protein adducts and complexes in plasma. Electrophoresis. 2008;29:682–694
  29. Gudehithlu KP, Pegoraro AA, Dunea G, Arruda JA, Singh AK. Degradation of albumin by the renal proximal tubule cells and the subsequent fate of its fragments. Kidney Int. 2004;65:2113–2122
  30. Comper WD, Hilliard LM, Nikolic-Paterson DJ, Russo LM. Disease-dependent mechanisms of albuminuria (An invited review). Am J Physiol Renal Physiol. 2008;295:F1589–F1600
  31. Tirumalai RS, Chan KC, Prieto DA, Issaq HJ, Conrads TP, Veenstra TD. Characterization of the low molecular weight human serum proteome. Mol Cell Proteomics. 2003;2:1096–1103
  32. Birn H, Christensen EI. Renal albumin absorption in physiology and pathology. Kidney Int. 2006;69:440–449
  33. Vay D, Parodi M, Rolla R, et al. Circulating antibodies recognizing malondialdehyde-modified proteins in healthy subjects. Free Radic Biol Med. 2001;30:277–286
  34. Ishii T, Ito S, Kumazawa S, et al. Site-specific modification of positively-charged surfaces on human serum albumin by malondialdehyde. Biochem Biophys Res Commun. 2008;371:28–32
  35. Ahmed N, Dobler D, Dean M, Thornalley PJ. Peptide mapping identifies hotspot site of modification in human serum albumin by methylglyoxal involved in ligand binding and esterase activity. J Biol Chem. 2005;280:5724–5732
  36. Cohen MP, Ziyadeh FN, Chen S. Amadori-modified glycated serum proteins and accelerated atherosclerosis in diabetes: pathogenic and therapeutic implications. J Lab Clin Med. 2006;147:211–219
  37. Ziyadeh FN, Wolf G. Pathogenesis of the podocytopathy and proteinuria in diabetic glomerulopathy. Curr Diabetes Rev. 2008;4:39–45

 Originally published online as doi: 10.1053/j.ajkd.2009.07.011 on September 27, 2009.

PII: S0272-6386(09)00987-1

doi: 10.1053/j.ajkd.2009.07.011

American Journal of Kidney Diseases
Volume 54, Issue 5 , Pages 871-880 , November 2009